Paracrine Proangiogenic Function of Human Bone Marrow-Derived Mesenchymal Stem Cells Is Not Affected by Chronic Kidney Disease

Figure 4

EV characterization. (a) Immunoblot analysis of protein content of EVs versus whole cells. EVs contain the marker Flotillin-1 but do not contain nuclear or mitochondrial proteins. (b) Nanosight Particle Tracking Analysis shows that the nominal size of these vesicles is 159 nm. (c) Immunoblot showing Flotillin-1 content in various density fractions. Flotillin-1 positive EVs had a density of 1.1 g/mL.

(a)

(b)

(c)