Platelet-Rich Plasma Improves the Wound Healing Potential of Mesenchymal Stem Cells through Paracrine and Metabolism Alterations

Figure 3

PRP treatment stimulates the proangiogenic properties of hMADS cells in vitro. (a) ELISA quantification of VEGF and SDF1 in conditioned media from hMADS cells after 24 hours of culture in the absence (untreated) or the presence of at 5%, 10%, or 20% PRP. (b) Left panel: representative images of capillary structures formed by endothelial HUVEC cells following a 48-hour exposure with conditioned media from untreated hMADS cells or hMADS cells treated with 5%, 10%, or 20% PRP. Right panel: capillary branch number and length after a 24-hour exposure of HUVEC cells to conditioned media from untreated hMADS cells or hMADS cells treated with 5%, 10%, or 20% PRP. (c) Left panel: representative images of the edge of agarose beads showing migration of HUVEC cells from agarose drops following a 24-hour exposure with the different conditioned media (hMADS cells grown in basal medium in the absence of PRP (untreated) or in the presence of 5%, 10%, or 20% PRP). White lines delineate the edge of the agarose droplets. Right panel: migration area quantification of HUVEC cells following a 24-hour exposure with the different conditioned media from hMADS cells in the absence of PRP (untreated) and 5%, 10%, or 20% PRP. Data represent of independent experiments. , .

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