Platelet-Rich Plasma Improves the Wound Healing Potential of Mesenchymal Stem Cells through Paracrine and Metabolism Alterations

Figure 4

PRP treatment improves the survival and the proliferation of hMADS cells both in vitro and in vivo. (a) Relative survival of hMADS cells engrafted in combination with PRP in mouse wounds by reference to engrafted untreated hMADS cells, assessed by quantitative transcriptional expression of human SF3A1 gene, at day 1, day 3, and day 7. Data represent the of mice per group independent experiments. , . (b) Relative survival of H₂O₂-injured hMADS cells following exposure to 5%, 10%, or 20% PRP by reference to PRP-untreated H₂O₂-injured cells (untreated) assessed by Annexin V/7ADD staining and flow cytometry analysis. Data represent the of independent experiments. , , . (c) Relative human ki67 transcriptional expression in mouse wounds corresponding to the expression of hMADS cells engrafted in combination with PRP by reference to the expression of hMADS cells engrafted alone, at day 1, day 3, and day 7 postinjury. Results represent the obtained from at least mice per group.. (d) Relative ki67 transcriptional expression of hMADS cells following a 24-hour exposure to 5%, 10%, or 20% PRP by reference to hMADS cells grown in basal medium in the absence of PRP (untreated). Data represent the of independent experiments. , .

(a)

(b)

(c)

(d)