Depth-Dependent Cellular Response from Dental Bulk-Fill Resins in Human Dental Pulp Stem Cells

Figure 1

Schematic of the cytotoxicity test procedure with different depths of specimens from the light curing and results of cell viability. (a) Specimen preparation depending on the depth from the light and their extraction for the cytotoxicity test against human dental pulp stem cells (hDPSCs). (b-e) The results of the WST cell viability assay, which was dependent on the product (SDR, VBF, BBF (bulk-fill resins), and ZFF (conventional flowable resin)) and specimen depth (top (0-2 mm), middle (2-4 mm), and bottom (4-6 mm)), are shown in (b-e). The bottom samples showed the most cytotoxicity among the three compartments (top, middle, and bottom) in all groups. Compared with SDR and VBF, BBF and ZFF showed more cytotoxicity. Different letters indicate significant differences between groups (, ).