Medium Perfusion Flow Improves Osteogenic Commitment of Human Stromal Cells

Figure 6

Effect of osteogenic induction on gene expression comparing standard static and dynamic cell culture conditions. qPCR analysis of osteogenic markers (BGLAP, ALPL, SPP1, RUNX2, and COL1A1) was performed on L88/5 cells stimulated with the osteogenic cocktail (20 nM 1α,25-dihydroxyvitamin D3, 50 μM L-ascorbic acid 2-phosphate, and 10 mM β-glycerophosphate) for 2 and 4 days under static and dynamic cell culture conditions. B2M and GAPDH were used as reference genes (2^-ΔΔCT method). Fold changes from control untreated cells at day 0 were calculated. All data are reported as mean±SEM of three biological replicates. and .

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