Evaluation of Chitosan Hydrogel for Sustained Delivery of VEGF for Odontogenic Differentiation of Dental Pulp Stem Cells

Figure 5

VEGF promoted odontogenic differentiation in DPSCs. The induced ALPase activity was tested in DPSCs treated with VEGF compared to cells without VEGF (a). VEGF treatment at 10 ng/ml concentration produced more mineralized nodules than 5 ng/ml VEGF treatment, and cells with 10 ng/ml, 50 ng/ml, and 100 ng/ml did not show an obvious difference in the amount of mineralized nodules (b). The quantitative analysis of ARS was conducted (c). NC: base culture medium; OM: odontogenic culture medium.

(a)

(b)

(c)