Pretreatment with G-CSF Could Enhance the Antifibrotic Effect of BM-MSCs on Pulmonary Fibrosis

Figure 3

G-CSF promotes CXCR4 expression in BM-MSCs in vitro. The BM-MSCs were treated with G-CSF for 24 h (a–d). (a) The proliferation of BM-MSCs was determined by CCK-8. (b) The mRNA expression of VLA-4, ICAM-1, and VCAM-1 was measured by real-time PCR. (c) The mRNA expression of CXCR4 and CXCR7 was determined by real-time PCR. (d) The population of CXCR4-expressing BM-MSCs was quantified by FCM. (e) Western blot analysis of AKT phosphorylation in BM-MSCs at 0, 10, 30, 60, and 240 min after G-CSF treatment. The BM-MSCs were pretreated with LY294002 for 2 h followed by treatment with G-CSF for 24 h (f–g). (f) The mRNA expression of CXCR4 in BM-MSCs was measured by real-time PCR. (g) Representative FCM analysis of CXCR4-expressing BM-MSC population. (h) Data analysis of CXCR4-expressing BM-MSC population tested by FCM. –6, , . Bars: mean ± SD.

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