Research Article

Stepwise Adipogenesis of Decellularized Cellular Extracellular Matrix Regulates Adipose Tissue-Derived Stem Cell Migration and Differentiation

Figure 2

Confirmation of decellularization of ASC ECM. (a) DAPI staining. (b) DNA content was measured to confirm removal of nuclei. GM: growth media; AD: adipogenic media. (c) Western blot of GAPDH was performed to confirm removal of cell components. (d) Immunostaining analysis of ECM protein contents after decellularization. FN: fibronectin; LM: laminin. (e) Total protein content before and after decellularization was measured using the BCA assay. Total VEGF and bFGF contents were analyzed by ELISA. (f) VEGF. (g) bFGF. Results are presented as the ; Student’s -test. and , compared with two groups. .
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