Stepwise Adipogenesis of Decellularized Cellular Extracellular Matrix Regulates Adipose Tissue-Derived Stem Cell Migration and Differentiation

<div>Cellular morphology and proliferation of ASCs cultured on different substrates. (a) Cellular morphology of ASCs cultured on different substrates. ASCs were reseeded on three different substrates: growth d-ECM, adipogenic d-ECM, and tissue culture polystyrene (TCP). Immunostaining for F-actin. (b) ASC morphology is quantitatively compared by measuring cell aspect ratio using ImageJ software. (c) EdU assay for proliferation of ASCs. (d) Quantitative analysis of EdU<sup>+</sup> cell proliferation rate. (e) Colony-forming assay. (f) Quantitative analysis of colony number per well. 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Stem Cells International

fig3

Figure 3

Figure 3: Stepwise Adipogenesis of Decellularized Cellular Extracellular Matrix Regulates Adipose Tissue-Derived Stem Cell Migration and Differentiation 

Figure 3 | Stepwise Adipogenesis of Decellularized Cellular Extracellular Matrix Regulates Adipose Tissue-Derived Stem Cell Migration and Differentiation 