Shear Stress Promotes Arterial Endothelium-Oriented Differentiation of Mouse-Induced Pluripotent Stem Cells

<div>Effect of shear stress on arterial or venous marker expression and NO secretion in iPSCs induced by growth factors. (a) Gene expression of arterial markers EphrinB2 and Neuropilin-1 evaluated by RT-PCR analysis. The expression of each gene was normalized based on the expression of GAPDH. (b) Gene expression of venous markers EphB4 and COUP-TF II evaluated by RT-PCR analysis. The expression of each gene was normalized based on the expression of GAPDH. (c) Protein expression of EphrinB2 and Neuropilin-1 detected by western blotting. (d) Nitric oxide production of shear-conditioned iPSCs. 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Stem Cells International

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Figure 5

Figure 5: Shear Stress Promotes Arterial Endothelium-Oriented Differentiation of Mouse-Induced Pluripotent Stem Cells 