FoxO1 Overexpression Ameliorates TNF-α-Induced Oxidative Damage and Promotes Osteogenesis of Human Periodontal Ligament Stem Cells via Antioxidant Defense Activation

Figure 1

FoxO1 overexpression increased the antioxidant potential of hPDLSCs. PDLSC-pMSCV-empty (NC) and PDLSC-pMSCV-FoxO1 (ov-FoxO1) were treated with 0 or 10 ng/mL TNF-α for 12 h. Cellular ROS generation following different treatments was measured by staining with DCFH-DA (a, b). MDA levels in hPDLSCs were determined by a Lipid Peroxidation MDA Assay Kit following treatment with α-MEM or TNF-α (10 ng/mL) for 72 h (c). The expression of MnSOD and catalase in hPDLSCs exposed to 0 or 10 ng/mL TNF-α for 72 h was determined by Western blotting (d). ; ; .

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