Potential Role of the Resident Mesenchymal Stem-Like Cells in Renal Fibrogenesis after Ureteral Obstruction

Figure 4

Phenotype characterization of ex vivo expanded cells. (a) Representative histograms of flow cytometry analysis show the expressions of CD90, CD106, CD73, CD45, CD34, CD4, MHC II, and FSP1 in the twice-passaged cells expanded from renal tissues at 7 days after ureteral obstruction. (b) Representative images of immunofluorescent staining on the adipogenic (FABP4-positive, red), ostogenenic (osteopontin-positive, red), and chondrogenic (collagen II-positive, red) differentiations from the twice-passaged cells. Nuclei are counterstained with 4,6-diamidino-2-phenylindole (DAPI, blue).

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