MicroRNA-150 Modulates Adipogenic Differentiation of Adipose-Derived Stem Cells by Targeting Notch3

Figure 2

miR-150 deficiency attenuated adipogenic differentiation in ADSCs. (a) Real-time PCR analysis of miR-150 in ADSCs of WT and miR-150 KO mice. U6 was used as the internal control. vs. WT mice. (b) Lipid formation assessed by oil red O staining after 7 days of adipogenic induction. Scale bar, 50 μm. (c) Quantification of lipid accumulation (oil red O staining density). vs. the WT mice. (d) Western blot analysis of PPAR-γ2 expression in ADSCs. (e) Quantification of PPAR-γ2 expression in ADSCs. Results were normalized with β-actin. and vs. the WT mice. independent experiments.

(a)

(b)

(c)

(d)

(e)