MicroRNA-150 Modulates Adipogenic Differentiation of Adipose-Derived Stem Cells by Targeting Notch3

Figure 4

miR-150 inhibited Notch3 expression by targeting its 3UTR. (a) Complementary of miR-150 seed sequence to the 3UTR of Notch3. miR-150 complementary sequence within Notch3 3UTR or its mutant sequence was inserted into the downstream of the luciferase construct. (b) Luciferase activity assay in ADSCs. Effects of miR-150 on luciferase activity of the reporter gene bearing wild-type or mutant 3UTR of Notch3 in ADSCs. (c) Western blot analysis of Notch3 expression in ADSCs of WT and miR-150 KO mice. (d) Western blot analysis of Notch3 expression in ADSCs with miR-150 overexpression. Results were normalized with β-actin. and vs. the control group. independent experiments.

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