MicroRNA-150 Modulates Adipogenic Differentiation of Adipose-Derived Stem Cells by Targeting Notch3

<div>Notch3 depletion attenuated adipogenic differentiation in ADSCs. (a) ADSCs were infected with the lentiviruses and selected with puromycin for 1 week. The shRNA mediated knockdown of Notch3 in ADSCs. Bar, 200 <i>μ</i>m. (b) Western blot analysis of Notch3 expression in ADSCs. Results were normalized with <i>β</i>-actin. (c) Lipid formation assessed by oil red O staining after 7 days of adipogenic induction. Bar, 100 <i>μ</i>m. (d) Quantification of lipid accumulation (oil red O staining density). (e) Western blot analysis of PPAR-<i>γ</i>2 and C/EBP<i>α</i> expression in ADSCs. (f) Quantification of PPAR-<i>γ</i>2 and C/EBP<i>α</i> expression. Results were normalized with <i>β</i>-actin. <svg height="10.7395pt" id="M34" style="vertical-align:-0.6370001pt" version="1.1" viewbox="-0.0498162 -10.1025 56.4993 10.7395" width="56.4993pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M486 158C486 177 478 202 466 220C413 228 386 236 336 262C386 288 413 297 466 304C478 323 486 347 485 366C470 376 444 381 422 380C389 338 368 319 321 288C323 345 329 372 349 422C339 442 322 461 305 470C289 461 271 442 262 422C281 372 287 345 290 288C243 319 222 338 189 380C167 381 142 376 125 366C125 347 133 322 145 304C198 296 225 288 275 262C225 236 198 227 145 220C133 201 125 177 126 158C141 148 167 143 189 144C222 186 243 205 290 236C288 179 282 152 262 102C272 82 289 63 306 54C322 63 340 82 350 102C330 152 324 179 321 236C368 205 390 186 422 144C444 143 470 148 486 158Z"></path></g><g transform="matrix(.0091,0,0,-0.0091,5.56,-5.741)"><path d="M486 158C486 177 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602Z"></path></g><g transform="matrix(.013,0,0,-0.013,46.402,0)"><path d="M113 -12C146 -12 170 11 170 46C170 78 146 103 114 103S58 78 58 46C58 11 82 -12 113 -12Z"></path></g><g transform="matrix(.013,0,0,-0.013,49.366,0)"><path d="M241 635C89 635 35 457 35 312C35 153 89 -12 240 -12C390 -12 443 166 443 312C443 466 390 635 241 635ZM238 602C329 602 354 454 354 312C354 172 330 22 240 22C152 22 124 173 124 313S148 602 238 602Z"></path></g><g transform="matrix(.013,0,0,-0.013,55.606,0)"><path d="M241 635C89 635 35 457 35 312C35 153 89 -12 240 -12C390 -12 443 166 443 312C443 466 390 635 241 635ZM238 602C329 602 354 454 354 312C354 172 330 22 240 22C152 22 124 173 124 313S148 602 238 602Z"></path></g><g transform="matrix(.013,0,0,-0.013,61.846,0)"><path d="M384 0V27C293 34 287 42 287 114V635C232 613 172 594 109 583V559L157 557C201 555 205 550 205 499V114C205 42 199 34 109 27V0H384Z"></path></g></svg> vs. the Sh-Vector group. <svg height="8.55521pt" id="M36" style="vertical-align:-0.2063904pt" 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Stem Cells International

fig5

Figure 5

Figure 5: MicroRNA-150 Modulates Adipogenic Differentiation of Adipose-Derived Stem Cells by Targeting Notch3 