MicroRNA-150 Modulates Adipogenic Differentiation of Adipose-Derived Stem Cells by Targeting Notch3

Figure 6

miR-150 increased adipogenic differentiation in hADSCs. (a) ADSCs were infected with the adv-miR-150 for 2 days. Bar, 200 μm. (b) miR-150 levels were determined using real-time polymerase chain reaction (PCR). (c) Western blot analysis of Notch3 expression in ADSCs. (d) Results were normalized with β-actin. (e) Lipid formation assessed by oil red O staining after 7 days of adipogenic induction. Bar, 50 μm. (f) Quantification of lipid accumulation (oil red O staining density). (g) Western blot analysis of PPAR-γ and C/EBPα expression in ADSCs. (h) Results were standardized to β-actin. , , vs. the GFP group. independent experiments.

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