Notch Signaling Inhibition by LY411575 Attenuates Osteoblast Differentiation and Decreased Ectopic Bone Formation Capacity of Human Skeletal (Mesenchymal) Stem Cells

<div>LY411575 affects multiple genetic pathways in hBMSCs. (a) Heat map and unsupervised hierarchical clustering performed on differentially expressed genes during osteoblastic differentiation of LY411575-treated hBMSCs compared to DMSO-treated control cells. (b) Bar chart illustrating the distribution of the top twenty enriched genetic pathways enriched in the significantly downregulated genes identified in LY411575-treated hBMSCs compared to DMSO-treated control cells. (c) Validation of a selected panel of downregulated genes in LY411575-treated hBMSCs compared to DMSO-treated control using qRT-PCR. Gene expression was normalized to <i>β</i>-actin. 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Stem Cells International

fig3

Figure 3

Figure 3: Notch Signaling Inhibition by LY411575 Attenuates Osteoblast Differentiation and Decreased Ectopic Bone Formation Capacity of Human Skeletal (Mesenchymal) Stem Cells 

Figure 3 | Notch Signaling Inhibition by LY411575 Attenuates Osteoblast Differentiation and Decreased Ectopic Bone Formation Capacity of Human Skeletal (Mesenchymal) Stem Cells 