Pyridoxal-5-Phosphate Promotes Immunomodulatory Function of Adipose-Derived Mesenchymal Stem Cells through Indoleamine 2,3-Dioxygenase-1 and TLR4/NF-κB Pathway

Table 1

Differentiation culture medium.

Medium	Composition
Osteogenesis	Low-glucose DMEM (Gibco, USA)
	10% FBS (Gibco, USA)
	0.1 μM dexamethasone (Solarbio, China)
	50 mM β-glycerol phosphate (Solarbio, China)
	0.2 mM ascorbic acid (Sigma-Aldrich, USA)
Adipogenesis	High-glucose DMEM (Gibco, USA)
	10% FBS (Gibco, USA)
	0.25 mM 3-isobutyl-1-methylxanthine (Solarbio, China)
	0.1 μM dexamethasone (Solarbio, China)
	0.1 mM indomethacin (Sigma-Aldrich, USA); 6.25 μg/mL insulin (PeproTech, UK)
Chondrogenesis	High-glucose DMEM (Gibco, USA)
	10% FBS (Gibco, USA)
	100 mg/mL sodium pyruvate (Solarbio, China)
	0.5 μM dexamethasone (Solarbio, China)
	50 μg/mL ascorbic acid (Sigma-Aldrich, USA)
	50 μg/mL proline (Solarbio, China)
	10% insulin-transferrin-sodium selenite media supplement (ITS) (Sigma, USA)
	10 ng/mL transforming growth factor β (TGF-β) (GenScript, China)