miRNAs in Extracellular Vesicles from iPS-Derived Cardiac Progenitor Cells Effectively Reduce Fibrosis and Promote Angiogenesis in Infarcted Heart

Figure 4

CPC^ISX-9-derived EV promoted cardiomyocyte proliferation and angiogenesis after myocardial infarction (MI) in mice. (a) Representative image of Ki67-positive cardiomyocytes (cTnT positive) in EV-CPC^ISX-9-treated mouse hearts 30 days after MI. . (b) Quantitative estimate of proliferating cardiomyocytes as determined by Ki67 staining in the peri-infarct region 30 days after myocardial infarction. PBS group: cardiomyocytes from 3 hearts; EV-iPSC group: cardiomyocytes from 3 hearts; EV-CPC^ISX-9 group, cardiomyocytes from 3 hearts. vs. the PBS group, ; # vs. the EV-iPSC group, . (c) Representative images of arteriole density in the peri-infarct area 4 weeks after MI. Arterioles were identified by α-SMA-positive staining (green) of vascular structures. . (d) Quantitative analysis of arteriole density in different treatment groups. vs. the PBS group, ; # vs. the EV-iPSC group, , .

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