Complementary Embryonic and Adult Cell Populations Enhance Myocardial Repair in Rat Myocardial Injury Model

Figure 2

Biodistribution of injected cells using MION labeling and FISH, showing that cell injections do not increase vascular density: (a) Isl-1⁺ cells retain MION labeling, as shown by Prussian blue staining, 4 weeks following injection with cells infiltrating the scar tissue (between yellow dotted lines) suggesting participation in the repair process. (b) Male donor-derived CD90⁺ cells were detected 4-week post-MI by fluorescence in situ hybridization (FISH) for the Y chromosome shown in red (arrows). (c)–(g) Representative images from all cohorts of cardiac tissue sections stained for von Willebrand factor. (c) sham; (d) saline; (e) Isl-1⁺; (f) CD90⁺; (g) Isl-1⁺/CD90⁺). Scale bar: 200 μm. (h) Quantification of all the sections analyzed (saline: ; sham: ; Isl-1: ; CD90⁺: ; Isl-1⁺/CD90⁺: ).

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