Epsins Regulate Mouse Embryonic Stem Cell Exit from Pluripotency and Neural Commitment by Controlling Notch Activation

Figure 1

EPN1 and EPN2 are expressed in mESCs. (a) mESCs express EPN1 and EPN2. Western blot analysis shows EPN1 and EPN2 expression in mESCs. β-Actin was used as the loading control. (b, c) Representative immunofluorescence images of (b) EPN1 and EPN2 and (c) clathrin (heavy chain) localization in mESC: both EPNs and clathrin are localized as punctuated dots at the plasma membrane and in the cytoplasm, a pattern typical of endocytic proteins. Nuclei are counterstained with Hoechst 33258. For (a, b, and c), biologically independent experiments.

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