Novel Cryopreservation Approach Providing Off-the-Shelf Availability of Human Multipotent Mesenchymal Stromal Cells for Clinical Applications

Figure 1

Effect of CPS composition on postthaw MSC viability and recovery. (a) Cell viability after cryopreservation in α-MEM containing 0.2 M sucrose and 0-10% DMSO. (b) Cell viability after cryopreservation in PPP containing 0.2 M sucrose and 0-10% DMSO. (c) Cell recovery assessed by Alamar Blue test after cryopreservation in α-MEM or PPP containing 0.2 M sucrose and 0-10% DMSO. (d) Viability (FDA/EB staining) and morphology of MSCs before cryopreservation; (e) viability (FDA/EB staining) and morphology of MSCs after cryopreservation using PPP/0.2 M sucrose/1% DMSO mixture (PS1D) and subsequent 24 hrs culture. Data is significantly different () compared to fresh control; data is significantly different () compared to 0% DMSO; data is significantly different () compared to 10% DMSO; ^data is significantly different () compared to α-MEM group with the same DMSO concentration. Statistical analysis was performed by the Kruskal-Wallis test.

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