Research Article
Effects of Placenta-Derived Mesenchymal Stem Cells on the Particulate Matter-Induced Damages in Human Middle Ear Epithelial Cells
Table 1
Oligonucleotide primer sequences for quantitative reverse transcriptase polymerase chain reaction.
| | Gene | Primer sequence (forward) | Primer sequence (reverse) | Annealing temperature (°C) | Product size (bp) |
| | MUC5B | 5-GCCTACGAGGACTTCAACGT-3 | 5-CCTTGATGACCACACGGGTG-3 | 60 | 79 | | TNFα | 5-CCCATGTTGTAGCAAACCCT-3 | 5-TGAGGTACAGGCCCTCTGAT-3 | 60 | 132 | | IL1β | 5-CATTGCTCAAGTGTCTGAAGC-3 | 5-CATGGCCACAACAACTGACG-3 | 60 | 238 | | IL6 | 5-ACTCACCTCTTCAGAACGAATTG-3 | 5-CCATCTTTGGAAGGTTCAGGTTG-3 | 60 | 149 | | Cyclooxygenase 2 (COX2) | 5-CAAATTGCTGGCAGGGTTGC-3 | 5-AGGGCTTCAGCATAAAGCGT-3 | 60 | 139 | | PGE2 | 5-GAAGAAGGCCTTTGCCAACC-3 | 5-GACGAAGCCCAGGAAAAGGA-3 | 60 | 145 | | TGFβ | 5-GCAAGTGGACATCAACGGGT-3 | 5-TCCGTGGAGCTGAAGCAATA-3 | 60 | 174 | | VEGF | 5-TCCACCATGCCAAGTGGTC-3 | 5-GTCCACCAGGGTCTCGATTG-3 | 60 | 128 |
|
|
