Systemic Infusion of Expanded CD133⁺ Cells and Expanded CD133⁺ Cell-Derived EVs for the Treatment of Ischemic Cardiomyopathy in a Rat Model of AMI

Figure 4

Effect of expanded CD133⁺ cells and expanded CD133⁺ cell-derived EVs on infarct size and renal injury. (a) Representative Masson’s trichrome-stained section of hearts from each of the four groups. Blue staining is showing collagen deposition after myocardial infarction induction. (b) Echocardiographic evaluation of infarct size within each group before and after transplantation. (c) Comparison of the occurrence of renal tubular epithelial lesion between groups. Each circle in the graphic represents an animal. (d) Representative micrographs of renal histology from each of the four groups at day 28 after treatment administration. Arrow number 1 indicates evidence of renal necrotic cells flaking off to the tubular lumen, number 2 shows cell necrosis from the tubular wall, number 3 indicates evidence of renal tubules with Tamm-Horsfall (THP) protein, number 4 indicates vascular congestion, and number 5 indicates evidence of corpuscles while arrow number 6 shows preserved renal tubules in control healthy group. °: outlier value; EVs: transplanted with expanded CD133⁺cell-derived EVs; CD133⁺: transplanted with expanded CD133⁺ cells; vehicle: treated with PBS; control: healthy animals ( to 8 per group).

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