Mesenchymal Progenitors Derived from Different Locations in Long Bones Display Diverse Characteristics

Figure 1

Mesenchymal progenitors were isolated and identified. (a) The schematic diagram of isolating MPs from different regions of rat long bones. Detailed procedures were described in Materials and Methods. (b) P-MPs, E-MPs, and M-MPs showed similar morphologies. Scale bar, 200 μm. (c) Flow cytometric analysis was used to detect the presence of common MP-associated cell surface antigens, and the proportions of CD90+CD34-CD45-CD11b- cells were evaluated. (d) Semiquantitative PCR and agarose gel electrophoresis analysis were used to detect the expression of other stem cell surface markers, and CD31 was a marker of endothelial cells.

(a)

(b)

(c)

(d)