Mesenchymal Progenitors Derived from Different Locations in Long Bones Display Diverse Characteristics

Figure 3

The bidirectional differentiation potential of different MPs was distinct. (a) After culturing for 21 days in osteogenic differentiation medium, alizarin red staining was performed. (b) Quantification of the mineralized matrix was measured. (c) The expression of osteoblast marker genes was detected by qRT-PCR. (d) After culturing for 14 days in adipogenic differentiation medium, oil red O staining was performed. Scale bar, 100 μm. (e) Quantification of intracellular oil red O was measured. (f) qRT-PCR was performed to detect the expression of adipocyte marker genes. Data was shown as . ; ; .

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