Coculture of hWJMSCs and pACs in Oriented Scaffold Enhances Hyaline Cartilage Regeneration In Vitro

Figure 1

hWJMSCs and pACs after three passages satisfied the characters of MSCs and hyaline cartilage, respectively. (a) hWJMSCs revealed spindles and a fibroblast-like shape, ×40. (b) Expression of surface markers on hWJMSCs by flow cytometry revealed that CD73, CD90, and CD105 were positive, while CD34, CD45, and HLA-DR were negative. (c) pAC morphology showed a polygonous or round-shaped form, ×40. (d) Immunofluorescence staining of collagen II (red) of pACs was much more intense than that of collagen I (green).

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