Research Article

Coculture of hWJMSCs and pACs in Oriented Scaffold Enhances Hyaline Cartilage Regeneration In Vitro

Figure 2

Coculture groups secreted abundant glycosaminoglycan and collagen II. (a) The gross morphology of hWJMSCs : pACs (100 : 0, 75 : 25, 50 : 50, 25 : 75, and 0 : 100). (b) H&E staining results. (c) Safranin “O” staining results. (d) The results of collagen I and collagen II immunofluorescence staining; 1 W: 1 week; 3 W: 3 weeks. (e) After coculturing for 3 weeks, western blotting analysis of collagen I, collagen II, and aggrecan; β-actin used as an internal reference protein. (f) The mean grey value of collagen I, collagen II, and aggrecan relative to that of β-actin. The results were the ; , .
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