Serum-Free Manufacturing of Mesenchymal Stem Cell Tissue Rings Using Human-Induced Pluripotent Stem Cells

Figure 1

Fabrication of hiPSC-MSC tissue rings. The hiPSCs were differentiated into NCSCs, which were then further differentiated into MSCs using a serum-free chemical-defined protocol. The PDMS devices were cast from 3D-printed ABS molds and coated with 10% pluronic acid before cell seeding. Passage #5–#10 hiPSC-MSCs were mixed with collagen scaffold and loaded into the PDMS devices to form the tissue rings surrounding the center posts. Scale bar: 1 mm.