Serum-Free Manufacturing of Mesenchymal Stem Cell Tissue Rings Using Human-Induced Pluripotent Stem Cells

Figure 3

Characterization of hiPSC-MSC tissue rings. (a) The shape of hiPSC-MSC tissue rings 24 hours after extraction fabricated with different collagen densities and different culture media. (b) hiPSC-MSC tissue rings had smaller inner diameter at 2 mg/mL collagen density compared to lower densities. (c) The ring diameter decreased with increase in collagen density. hiPSC-MSC tissue rings showed (d) smaller inner diameter and (e) comparable ring diameter, for serum-containing media and serum-free media. Scale bar: 1 mm.

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