Serum-Free Manufacturing of Mesenchymal Stem Cell Tissue Rings Using Human-Induced Pluripotent Stem Cells

Figure 5

Histochemistry of hiPSC-MSC tissue rings. (a) H&E staining of hiPSC-MSC tissue rings represented cytoplasm (pink) and nuclei (blue). Immunofluorescent staining of hiPSC-MSC tissue ring with (b) CD73 and (c) CD90 demonstrated that the tissue rings maintained the MSC biomarkers after the fabrication process. (d) The hiPSC-MSC tissue rings have been successfully differentiated into chondrogenic lineage, indicating that the tissue rings retained the potency for further differentiation. Scale bar: whole tissue structure (left) 500 μm and zoom-in close-ups (right) 10 μm.

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