Mobilization of Transplanted Bone Marrow Mesenchymal Stem Cells by Erythropoietin Facilitates the Reconstruction of Segmental Bone Defect

Figure 1

The characteristics of BMSCs. The passage 3 BMSC immunofluorescent labelings were positive for CD29 (a), CD44 (b), CD90 (c), and CD271 (d) and negative for CD11b (e) and CD45 (f). (g) BMSCs manifested typical spindle or polygonous shape and adhered to the plastic culture plate. (h) Positive oil red O staining on day 14 revealed the differentiation of passage 3 BMSCs into adipocytes. (i) ALP staining of passage 3 BMSCs indicated formation of calcification nodes on day 21. Scale bar is 100 μm for (a, c, g, h, and i) and 50 μm for (b, d, e, and f).

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