Isolation and Characterization of Neural Stem Cells from the Rat Inferior Colliculus

Figure 4

Neurosphere of the IC after 4 days of growth on a glass coverslip. After two days on PDL- and laminin-coated coverslips, these neurospheres showed an outgrowth of sphere branches with an increase in length over the course of time. Nuclei were stained with DAPI (blue). The neural progenitor cell marker Nestin (green) was positive in cells inside the sphere and in the branches. Progenitor cells within the neurospheres were stained positive for the neuronal migration protein doublecortin (DCX) (red).