Isolation and Characterization of Neural Stem Cells from the Rat Inferior Colliculus

Figure 7

Results of the immunocytochemical analysis of the plated single cells of IC NSC cultures from PND6 rats. The proportions of positively stained cells in relation to the total number of cells on coverslips were evaluated. The boxplots show mean and SEM. (a) Cell proportions that are positive for the analyzed progenitor cell markers after 24 hours on glass coverslips in NSC medium (b) Differentiated cells after 6 days on glass coverslips in DIF medium. Differentiated cell types could be identified morphologically and by immunohistochemical markers of the neuroectodermal lineage. β-III-Tubulin stains cells that differentiate into the neuronal lineage, GFAP stains those that differentiate into the astroglial lineage, and MBP stains those that differentiate into the oligodendrocyte lineage.

(a)

(b)