CD8⁺γδ T Cells Are More Frequent in CMV Seropositive Bone Marrow Grafts and Display Phenotype of an Adaptive Immune Response

Figure 6

Enhanced response of CD8⁺γδ T cells to TCR and cytokine stimulation. (a) representative histograms () of proliferating T cells from unstimulated or TCR/CD3 stimulated condition, gated on TCR γδ^- CD8⁺ (blue), CD8⁺γδ (green), and CD8^-γδ (orange). (b) Proportions of proliferating CD8⁺γδ (green) and CD8^-γδ (orange) cells after TCR/CD3 stimulation. (c) Representative FACS plot of TIM3, CD69, and KIR2DL2/L3 gated on CD8⁺γδ (upper) and CD8^-γδ (lower) T cells after TCR/CD3 stimulation. (d) Proportions of CD8⁺γδ T cells (blue) and CD8^-γδ T cells (red) expressing TIM3+, CD69+, and KIR2DL2/L3+ after TCR/CD3 stimulation. Paired -test used. (e) Representative histogram of proliferation of CD8⁺γδ (A) and CD8^-γδ (B) cultured in the presence of medium only (green), IL-7 (orange), IL-15 (blue), and IL-18 (red). Proportions of proliferating cells after culture in a medium, IL-7, IL-15, and IL-18 in CD8⁺γδ (f) and CD8^-γδ (g). Repeated measures ANOVA is used. Bar and whiskers represent the mean and S.E.M.

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