Microvesicles Derived from Human Embryonic Neural Stem Cells Inhibit the Apoptosis of HL-1 Cardiomyocytes by Promoting Autophagy and Regulating AKT and mTOR via Transporting HSP-70

Figure 1

Experimental groups. NC group: the cells were kept in a 37°C in humid air with 5% CO₂ incubator for the entire experiment. Pre-HSP-70 group: the cells were kept in a 42°C in humid air with 5% CO₂ incubator for 30 min. Triptolide+pre-HSP-70 group: the cells were treated with triptolide 1 h before heat shock preconditioning. H₂O₂ group: the cells were kept in 1000 mM H₂O₂ for 3 h. Tripolide+H₂O₂ group: the cells were treated with triptolide 1 h before H₂O₂ treated. Pre-HSP-70+H₂O₂ group: 8 h after heat shock preconditioning, the cells were treated with H₂O₂ 3 h. Tripolide+Pre-HSP-70+H₂O₂ group: 1 h after triptolide treatment, cells were heat shock preconditioned for 8 h and then followed by treatment with H₂O₂ for 3 h after the Pre-HSP-70 treatment.