Microvesicles Derived from Human Embryonic Neural Stem Cells Inhibit the Apoptosis of HL-1 Cardiomyocytes by Promoting Autophagy and Regulating AKT and mTOR via Transporting HSP-70

Figure 3

hESC-NSC-derived MVs reduced cell apoptosis after H₂O₂ stimulation. CCK-8 was used to measure HL-1 cell viability after exposure to 0, 500, 800, and 1000 μM H₂O₂ for 3 h (a). Representative western blot images showing the protein levels of Bcl-2, Bax, and cleaved caspase-3. β-Actin was used as an internal control (b–f). Representative dot plots of cell apoptosis were showed after Annexin V/PI dual staining (g). The percentage of apoptotic cells was represent for both early and late apoptotic cells (h). Every experiment was repeated at least three times; error bars indicate (; ; ).

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)