Microvesicles Derived from Human Embryonic Neural Stem Cells Inhibit the Apoptosis of HL-1 Cardiomyocytes by Promoting Autophagy and Regulating AKT and mTOR via Transporting HSP-70

Figure 4

hESC-NSC-derived MVs increased the level of cell autophagy after H₂O₂ stimulation. Representative western blot images showing the protein levels of LC3, Beclin-1, and P62. β-Actin was used as an internal control (a, b). Autophagosomes were detected by tandem fluorescent mRFG-GFP-LC3 assay, scale bars: 15 μm (c). Autophagosomes (white arrow) were measured by TEM in four groups, scale bars: 2 μm (d). Every experiment was repeated at least three times; error bars indicate (; ; ).

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