Microvesicles Derived from Human Embryonic Neural Stem Cells Inhibit the Apoptosis of HL-1 Cardiomyocytes by Promoting Autophagy and Regulating AKT and mTOR via Transporting HSP-70

Figure 5

Transfer of HSP-70 between cells via hESC-NSCs-derived MVs. Coomassie blue staining and western blot analysis of protein extracts from MVs (a). Western blot images showing the protein levels of the HSP-70 after HSPre with/without triptolide pretreatment (b–e). Western blot images showing the protein levels of the HSP-70 in HL-1 cells were exposed to 42°C for 10, 30, 60, and 120 min and then allowed to recover at 37°C for 0, 4, 8, 12, and 24 h. β-Actin was used as an internal control (f–h). Every experiment was repeated at least three times. Error bars indicate (; ; ).

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