Research Article
Microvesicles Derived from Human Embryonic Neural Stem Cells Inhibit the Apoptosis of HL-1 Cardiomyocytes by Promoting Autophagy and Regulating AKT and mTOR via Transporting HSP-70
Figure 7
HSP-70 increased the level of cell autophagy after H2O2 stimulation. (a–d) Representative western blot images showing the protein levels of LC3, Beclin-1, and P62. β-Actin was used as an internal control. (e) Autophagosomes (white arrow) were detected by tandem fluorescent mRFG-GFP-LC3 assay, scale bars: 15 μm. (f) Autophagosomes were measured by TEM in four groups. Every experiment was repeated at least three times, scale bars: 2 μm. Error bars indicate (; ; ).
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