Activator Protein-1 Transcriptional Activity Drives Soluble Micrograft-Mediated Cell Migration and Promotes the Matrix Remodeling Machinery

Figure 2

MG-dependent transcriptional and posttranscriptional regulation of AP-1 family members. (a) Transcript levels of AP-1 family members evaluated by RT-qPCR. Expression values are expressed as a fold change normalized to the expression of Gapdh, β-actin, and Rpl13a housekeeping genes. Differences are calculated with one-way ANOVA () and indicated as , , , and . (b) Representative immunofluorescence showing phosphorylation and total levels of Fra-1 protein in MG-treated and untreated primary adult fibroblasts. Nuclei were counterstained with Hoechst 33342 (). (c) Representative western blot membranes showing level of phosphorylated and total Fra-1 and c-Jun from wounded fibroblasts upon different time durations of MG treatment (1 h and 5 h). Untreated cells were used as a control. (d) Representative western blot membranes showing level of phosphorylated and total FRA-1 and c-JUN from MG-treated and MG-untreated human keratinocyte (HaCaT) cells exposed to 1 h and 5 h of MG treatment.

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