Research Article
Activator Protein-1 Transcriptional Activity Drives Soluble Micrograft-Mediated Cell Migration and Promotes the Matrix Remodeling Machinery
Figure 3
MG treatment accelerates scratch closure and cell migration through transcriptional activity of AP-1. (a) Representative images of wounded adult mouse fibroblasts exposed to 5 h of MG treatment in the presence or absence of the specific AP-1 inhibitor T-5224 (60 μM). (b) Quantification of scratch closure from 3 independent experiments as in Figure 3(a). Data is presented as the (). The surface area was normalized to the T0 image, and significant differences are calculated with two-way ANOVA, followed by the Holm-Sidak test and indicated as ; ; . (c) Representative images of wounded human keratinocytes exposed to 5 h and 12 h of MG treatment in the presence or absence of AP-1 inhibitor T-5224 (60 μM). (d, e) Quantification of scratch closure from 3 independent experiments as in Figure 3(c). Data is shown as the (). The surface area was normalized to the T0 image and significant differences are calculated by two-way ANOVA followed by the Tukey test and indicated as , , , and . (f, g) Pictures showing transwell migration assays chamber membranes. Adult murine fibroblasts (left panel) and human (HaCaT) keratinocytes (right panel) were exposed to MG treatment in the presence or absence of the AP-1 inhibitor T-5224 (60 μM). Data is presented as the . values were calculated using ordinary one-way ANOVA corrected by the Tukey test. , , , .
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