Activator Protein-1 Transcriptional Activity Drives Soluble Micrograft-Mediated Cell Migration and Promotes the Matrix Remodeling Machinery

Figure 6

ERK signaling pathway activation regulates wound healing events through AP-1 transcription factor activity. (a) Representative western blot showing protein levels of phosphorylated and total ERK, Fra-1, and c-Jun from wounded murine adult fibroblasts and unwounded human keratinocytes (HaCaT cells) exposed to 1 h and 5 h of MG treatment in the presence or absence of the MEK inhibitor PD0325901 (1 μM). (b) Principal component analysis (PCA) plot obtained from RNA-seq data indicates clustering between untreated and MG-treated samples with or without MEK inhibition. (c) Heatmaps showing gene expression levels of DEGs discovered through RNA-seq analysis are shown to be involved in essential wound healing related events, including cell migration, angiogenesis, cell cycle, and epithelialization. Gene expression levels of AP-1 family members are presented as heatmap in all the conditions under study.

(a)

(b)

(c)