Cancer Stem Cells from Tumor Cell Lines Activate the DNA Damage Response Pathway after Ionizing Radiation More Efficiently Than Noncancer Stem Cells

Figure 4

Cancer stem cells have baseline expression of pATM and γH2A.X. Immunofluorescence staining for pATM and γH2A.X protein (green). Visualization of nuclei by DAPI (blue). (a) HeLa SP cultures expressed pATM and γH2A.X in baseline levels. (b) MCF-7 SP cultures expressed pATM and γH2A.X in baseline levels. The representative images of the microscopy analysis indicate that pATM and γH2A.X proteins are expressed in baseline levels in CSC cultures. Objective 60x.