Phenotypic Analysis of BrdU Label-Retaining Cells during the Maturation of Conducting Airway Epithelium in a Porcine Lung

Figure 2

Composition of epithelial cell types in conducting airway epithelium of an adult porcine lung. Representative images of immunohistochemistry (IHC) staining with epithelial cell-specific markers were used for the identification of epithelial cell types of an adult porcine lung. (a, e, i) Immunohistochemistry staining of CCSP (brown) revealed that infrequent club cells are distributed in the region of the trachea, bronchus, and bronchiole. (b, f, j) Immunohistochemistry staining of K14 (brown) showed that basal cells are distributed in the region of the trachea, bronchus, and bronchiole. (c, g, k) Immunohistochemistry staining of T4 (brown) showed that ciliated cells are tapered along the tracheal-bronchial airway axis. (d, h, l) Immunohistochemistry staining of SPC (brown) showed the alveolar type 2 (AT2) cells in the alveoli region of a distal lung, counterstained with hematoxylin (blue). Bar = 50 or 100 μm.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)

(k)

(l)