Phenotypic Analysis of BrdU Label-Retaining Cells during the Maturation of Conducting Airway Epithelium in a Porcine Lung

Figure 3

Immunofluorescence staining of epithelial cell-specific markers in the middle trachea of an adult porcine lung. Representative images of immunohistochemistry (IHC) staining with epithelial cell-specific markers were used for the identification of epithelial cell types in the middle trachea of an adult porcine lung. (a, b, c) Immunofluorescence staining for keratins of porcine tracheal epithelium, (a) pan keratin (green); (b) keratin 5 (Krt5) and (c) keratin 14 (Krt14) showed that basal cells were cuboidal-shaped and relatively short and contact the basement membrane and luminal cells. (d) Immunofluorescence staining for CCSP (green) revealed that infrequent cone-shaped club cells resided in the porcine tracheal epithelium. (e) Immunofluorescence staining for tubulin IV (green) showed the ciliated cells in the tip of epithelial layer. (f) Immunofluorescence staining for mucin 5AC (green) revealed that the secretory cells include morphologically and functionally distinct cells such as the goblet, serous, and club cells. Nuclear stained with DAPI (blue). Bar = 50 μm.

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