Phenotypic Analysis of BrdU Label-Retaining Cells during the Maturation of Conducting Airway Epithelium in a Porcine Lung

Figure 5

Efficiency of BrdU labeling in epithelial cells of a newborn porcine lung. The BrdU labeling efficiency was determined by immunofluorescence staining of BrdU and epithelial cell markers. Representative images of IF staining for colocalization of BrdU and epithelial cell type markers keratin 14, mucin 5AC, and ProSPC in the trachea and distal lung of porcine at day 1 post BruU pulse. (a, b) Colocalization of keratin 14 (green) and BrdU (red) in the surface airway epithelium (SAE) (a) and submucosal glands (SMG) (b) in the middle trachea of a porcine lung. (c, d) Colocalization of mucin 5AC (green) and BrdU (red) in the surface airway epithelium (SAE) (c) and submucosal glands (SMG) (d) in the middle trachea of a porcine lung. (e, f) Low (e) and high (f) magnifications of images of colocalization of ProSPC (green) and BrdU (red) in the alveolar region of a porcine distal lung. Nuclear stained with DAPI (blue). Bar = 50 μm in (a, c, e). Bar = 100 μm in (b, d, f).

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