Suppressing MicroRNA-30b by Estrogen Promotes Osteogenesis in Bone Marrow Mesenchymal Stem Cells

Figure 4

The effect of E2 on BMP2 expression. (a) Western blotting shows that the expression of BMP2 was increased with 10 nM and 100 nM E2 stimulation for 5 days. The expression of BMP2 was also increased with 1 nM, 10 nM, and 100 nM E2 stimulation for 9 days. (b–c) The relative protein levels were calculated based on the loading control for day 5 (b) and day 9 (c). CM: complete medium; OM: osteogenic medium. Three independent experiments were performed. (OM group vs. 1-100 nM E2 group, .)

(a)

(b)

(c)