Genes Involved in the Transcriptional Regulation of Pluripotency Are Expressed in Malignant Tumors of the Uterine Cervix and Can Induce Tumorigenic Capacity in a Nontumorigenic Cell Line

Figure 3

The mRNA levels of OCT4, SOX2, and NANOG are high in cancer stem cell-enriched cultures. The messenger RNA levels of OCT4, SOX2, and NANOG are higher in the cancer stem cell-enriched cultures grown as spheres from HeLa (HeLa SP) and SiHa (SiHa SP) cells than in their monolayer cultures (HeLa and SiHa, respectively) grown conventionally. Experiments were performed in triplicate, and the values are expressed as . Beta2-microglobulin (β2M) was used as the housekeeping gene. value < 0.05, value < 0.01, and value < 0.0001.

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