Genes Involved in the Transcriptional Regulation of Pluripotency Are Expressed in Malignant Tumors of the Uterine Cervix and Can Induce Tumorigenic Capacity in a Nontumorigenic Cell Line

Table 1

In vivo tumorigenic properties of OCT4, SOX2, KLF4, C-MYC, and NANOG.

Condition	Tumor size	Mice with tumor
HeLa	++++	6/6
HaCaT	-	0/6
HaCaT+EGFP	-	0/6
HaCaT+OCT4	+	6/6
HaCaT+SOX2	++	6/6
HaCaT+NANOG	+++	6/6
HaCaT+KLF4	++	6/6
HaCaT+C-MYC	++	6/6
HaCaT+OSKM-N	+++	6/6
HaCaT+SOX2+NANOG	+++	6/6

HaCaT cells were infected with EGFP-, OCT4-, SOX2-, KLF4-, C-MYC-, and NANOG lentiviruses, cultured and puromycin-selected for 1 month, and then injected into NOD-SCID female mice ( for each experimental condition). The + symbol represents positive tumor generation within a period of 7 weeks. HaCaT and HeLa cells were utilized as negative and positive controls, respectively. The results of these experiments indicated that the overexpression of OCT4, SOX2, KLF4, C-MYC, and NANOG taken together was associated with significant tumor growth in HaCaT cells. The number of tumors formed and the number of inoculations performed are indicated for each condition as a fractional number. + represents the size of the tumors: the greater the number of symbols, the larger the tumor size. - represents the absence or nonformation of a tumor.